Hotspot

rps4

LSC · 46047-46652 · 606 bp

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Pi 0.00495

MarkerSeek score 61.60

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 3
pi: 0.00495
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGGGGAAATTTGTACGAGCC	ACAATACTTCAGCGGATTTCG	50.0	42.9	57.9	56.7	735 bp	1.0	61.0
rps4_p2	GGGAAATTTGTACGAGCCCA	ACAATACTTCAGCGGATTTCG	50.0	42.9	58.2	56.7	733 bp	1.0	60.7
rps4_p3	CCCGGATCAAACTCCTTACC	ACAATACTTCAGCGGATTTCG	55.0	42.9	57.7	56.7	783 bp	1.0	59.4
rps4_p4	GGGGAAATTTGTACGAGCCC	ACAATACTTCAGCGGATTTCG	55.0	42.9	58.9	56.7	734 bp	1.0	54.8
rps4_p5	AGGGGAAATTTGTACGAGCC	GTACAATACTTCAGCGGATTTCG	50.0	43.5	57.9	57.9	737 bp	1.0	54.8