Hotspot

ndhH

SSC · 123681-124862 · 1182 bp

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Pi 0.00169

MarkerSeek score 59.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.00169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCACCCACAAGATCCCATAG	AGAATGAATCGAGGAAAAACC	55.0	38.1	58.0	54.2	1293 bp	1.0	41.4
ndhH_p2	GGAGTCGAAATAGGGGCAAG	AGAATGAATCGAGGAAAAACC	55.0	38.1	58.0	54.2	1312 bp	1.0	41.2
ndhH_p3	GCACCCACAAGATCCCATAG	AAGAATGAATCGAGGAAAAACC	55.0	36.4	58.0	55.0	1294 bp	1.0	40.1
ndhH_p4	GGAGTCGAAATAGGGGCAAG	AAGAATGAATCGAGGAAAAACC	55.0	36.4	58.0	55.0	1313 bp	1.0	40.1
ndhH_p5	GCACCCACAAGATCCCATAG	TGTAAGAATGAATCGAGGAAAAACC	55.0	36.0	58.0	57.9	1297 bp	1.0	40.1