Hotspot

rpl32-trnL-UAG

SSC · 114743-115756 · 1014 bp

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Pi 0.00197
MarkerSeek score 60.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1014
variable_sites
2
pi
0.00197
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CAGCGTTGAAGGCTTTTTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.5 58.0 1147-1149 bp 1.0 76.2
rpl32-trnL-UAG_p2 CAGCGTTGAAGGCTTTTTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.5 58.0 1148-1150 bp 1.0 76.2
rpl32-trnL-UAG_p3 CAGCGTTGAAGGCTTTTTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.5 57.9 1157-1159 bp 1.0 75.3
rpl32-trnL-UAG_p4 CAGCGTTGAAGGCTTTTTCA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.5 57.8 1156-1158 bp 1.0 74.7
rpl32-trnL-UAG_p5 CAGCGTTGAAGGCTTTTTCA CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.5 57.8 1155-1157 bp 1.0 74.7