Hotspot

ycf15-trnL-CAA

IRb · 94427-95151 · 725 bp

Back to Dictamnus

Pi 0.00552
MarkerSeek score 61.90
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
725
variable_sites
4
pi
0.00552
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 GCGAACCACCAGAGCTATTA AAATGGTAGACACGCGAGAC 50.0 50.0 57.4 58.0 842 bp 1.0 75.5
ycf15-trnL-CAA_p2 GCGAACCACCAGAGCTATTA TGAAATGGTAGACACGCGAG 50.0 50.0 57.4 58.0 844 bp 1.0 75.5
ycf15-trnL-CAA_p3 GCGAACCACCAGAGCTATTA GCCTTGGTGGTGAAATGGTA 50.0 50.0 57.4 58.1 854 bp 1.0 74.9
ycf15-trnL-CAA_p4 TTCTTGAACAGCGAACCACC AAATGGTAGACACGCGAGAC 50.0 50.0 58.7 58.0 852 bp 1.0 74.8
ycf15-trnL-CAA_p5 TTCTTGAACAGCGAACCACC TGAAATGGTAGACACGCGAG 50.0 50.0 58.7 58.0 854 bp 1.0 74.7