Hotspot

psaJ-rpl33

LSC · 68021-68514 · 494 bp

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Pi 0.0061

MarkerSeek score 60.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 3
pi: 0.0061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	GGACATCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	634-636 bp	1.0	78.4
psaJ-rpl33_p2	TCGGGTCTTTAGCAGGGTTA	GGACATCTTTACCCTTGGCC	50.0	55.0	58.1	58.2	594-596 bp	1.0	78.3
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	ACACCGTTTCGAACACAACT	50.0	45.0	58.0	58.3	680-682 bp	1.0	78.1
psaJ-rpl33_p4	TCGGGTCTTTAGCAGGGTTA	ACACCGTTTCGAACACAACT	50.0	45.0	58.1	58.3	640-642 bp	1.0	78.0
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	GGACATCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	621-623 bp	1.0	77.0