Hotspot

ndhC-trnV-UAC

LSC · 51281-52206 · 926 bp

Back to Dictamnus

Pi 0.00216
MarkerSeek score 60.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
926
variable_sites
2
pi
0.00216
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGTTAACCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1068-1069 bp 1.0 77.0
ndhC-trnV-UAC_p2 TTAACCGGGGCTAAAACTGC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1062-1063 bp 1.0 74.9
ndhC-trnV-UAC_p3 CCTTTGTTAACCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1067-1068 bp 1.0 74.3
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGTTAACC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.4 57.8 1076-1077 bp 1.0 74.0
ndhC-trnV-UAC_p5 CCTTTGTTAACCGGGGCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.8 58.7 1056-1057 bp 1.0 73.1