Pi
0
MarkerSeek score
61.00
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CAACTAGAGAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1036-1058 bp | 1.0 | 80.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 981-1003 bp | 1.0 | 80.7 |
| trnT-UGU-trnL-UAA_p3 | CAACTAGAGAAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.9 | 58.1 | 1044-1066 bp | 1.0 | 80.3 |
| trnT-UGU-trnL-UAA_p4 | ACAACTAGAGAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 1037-1059 bp | 1.0 | 80.1 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 989-1011 bp | 1.0 | 80.1 |