Hotspot

psaA-ycf3

LSC · 42664-43365 · 702 bp

Back to Dictamnus

Pi 0.0057

MarkerSeek score 62.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 4
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	814 bp	1.0	78.4
psaA-ycf3_p2	TTGAGAAATGACCCGGTCTG	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.5	57.9	834 bp	1.0	75.6
psaA-ycf3_p3	TTTTACTTCTGGTTCCGGCG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.5	57.9	767 bp	1.0	75.5
psaA-ycf3_p4	GGCCCATTCCTCGAAAGAAG	ATTGGTTGAAGATCACGGGG	55.0	50.0	58.6	57.9	815 bp	1.0	74.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TCAAGCCCAGAATTGGTTGA	50.0	45.0	57.9	57.3	825 bp	1.0	74.1