Hotspot

trnE-UUC-trnT-GGU

LSC · 31265-32097 · 833 bp

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Pi 0

MarkerSeek score 60.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	927-938 bp	1.0	78.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	906-917 bp	1.0	78.7
trnE-UUC-trnT-GGU_p3	TTCCTAGTACCCTACCCCCA	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	964-975 bp	1.0	78.5
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCAAAAAGCCCCTTATCGGA	55.0	50.0	58.3	57.9	950-961 bp	1.0	77.7
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	924-935 bp	1.0	77.6