Hotspot

atpH-atpI

LSC · 13746-14528 · 783 bp

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Pi 0.00255

MarkerSeek score 60.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 2
pi: 0.00255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	870 bp	1.0	78.8
atpH-atpI_p2	GATCCCTTCTACTGCTTGGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.0	57.9	955 bp	1.0	78.8
atpH-atpI_p3	GTACCTTGACCAACACCAGG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.1	57.9	927 bp	1.0	78.2
atpH-atpI_p4	GTCCAATAGAAGCAAGCCCA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	908 bp	1.0	78.1
atpH-atpI_p5	AACGGAAGCGGCAGAAATAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	868 bp	1.0	77.7