Hotspot

trnK-UUU-rps16

LSC · 4377-4926 · 550 bp

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Pi 0.00545

MarkerSeek score 60.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 550
variable_sites: 3
pi: 0.00545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGCCGTACTTAAAAGCCGAG	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.0	58.0	655-656 bp	1.0	80.2
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	644-645 bp	1.0	78.9
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	643-644 bp	1.0	78.9
trnK-UUU-rps16_p4	AGCCGTACTTAAAAGCCGAG	CCAACCTACAGGAACCGTTC	50.0	55.0	58.0	58.2	680-681 bp	1.0	78.7
trnK-UUU-rps16_p5	AGCCGTACTTAAAAGCCGAG	TCAAAGAAAGCGGGGGTTTT	50.0	45.0	58.0	58.2	652-653 bp	1.0	78.6