Hotspot

rps3

Genome · 2881-3537 · 657 bp

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Pi 0.00609

MarkerSeek score 61.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: Genome
length_bp: 657
variable_sites: 4
pi: 0.00609
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	AGCCCAAGGACGAGGATATC	GGTCGCACACTAAGCATAAC	55.0	50.0	58.7	56.9	890 bp	1.0	66.4
rps3_p2	GAGCCCAAGGACGAGGATAT	GGTCGCACACTAAGCATAAC	55.0	50.0	58.7	56.9	891 bp	1.0	66.4
rps3_p3	GAGCCCAAGGACGAGGATA	GGTCGCACACTAAGCATAAC	57.9	50.0	58.2	56.9	891 bp	1.0	62.2
rps3_p4	AGCCCAAGGACGAGGATATC	GGTCGCACACTAAGCATAACA	55.0	47.6	58.7	58.7	890 bp	1.0	61.8
rps3_p5	GAGCCCAAGGACGAGGATAT	GGTCGCACACTAAGCATAACA	55.0	47.6	58.7	58.7	891 bp	1.0	61.8