Hotspot

ndhH

Genome · 103651-104826 · 1176 bp

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Pi 0.0034

MarkerSeek score 60.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1176
variable_sites: 4
pi: 0.0034
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTGA	35.0	39.1	53.8	56.9	1326 bp	1.0	40.2
ndhH_p2	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTGAA	35.0	37.5	53.8	57.5	1326 bp	1.0	40.2
ndhH_p3	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTG	35.0	40.9	53.8	55.4	1326 bp	1.0	40.2
ndhH_p4	ATGAATGAAAAGGAGCGTTA	GCTAACAAGAGAATGGTGATTGA	35.0	39.1	52.9	56.9	1325 bp	1.0	40.2
ndhH_p5	TGAATGAAAAGGAGCGTTAT	GCTAACAAGAGAATGGTGATTGA	35.0	39.1	52.9	56.9	1324 bp	1.0	40.2