Hotspot

psbM-trnL-CAA

Genome · 81859-82084 · 226 bp

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Pi 0

MarkerSeek score 24.10

Primer available yes

Label psbM-trnL-CAA

Feature Metadata

label_name: psbM-trnL-CAA
feature_type: igs
region: Genome
length_bp: 226
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnL-CAA_p1	TTAGTGAAAAGGCTGTGGGT	AGGCCTTGATGGTGAAATGG	45.0	50.0	57.0	58.1	379 bp	1.0	70.5
psbM-trnL-CAA_p2	AGTGAAAAGGCTGTGGGTAT	AGGCCTTGATGGTGAAATGG	45.0	50.0	56.7	58.1	377 bp	1.0	68.6
psbM-trnL-CAA_p3	TTAGTGAAAAGGCTGTGGGT	AACAGGCCTTGATGGTGAAA	45.0	45.0	57.0	57.6	382 bp	1.0	68.6
psbM-trnL-CAA_p4	TTAGTGAAAAGGCTGTGGGT	GGCCTTGATGGTGAAATGGT	45.0	50.0	57.0	58.4	378 bp	1.0	68.1
psbM-trnL-CAA_p5	TTAGTGAAAAGGCTGTGGGT	TTAAAGCGTGGAGGTTCGAA	45.0	45.0	57.0	57.5	330 bp	1.0	67.4