Hotspot

trnC-GCA-psbM

Genome · 80265-81750 · 1486 bp

Back to Dicranum

Pi 0.0128

MarkerSeek score 53.80

Primer available yes

Label trnC-GCA-psbM

Feature Metadata

label_name: trnC-GCA-psbM
feature_type: igs
region: Genome
length_bp: 1486
variable_sites: 19
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-psbM_p1	AGTTCAAATCTGGGTGTCGC	GCTAGTAGTCAGGGCGGTTA	50.0	55.0	58.5	58.6	1529 bp	1.0	72.4
trnC-GCA-psbM_p2	GATATGGCCAAGCGGTAAGG	GCTAGTAGTCAGGGCGGTTA	55.0	55.0	58.5	58.6	1575 bp	1.0	72.3
trnC-GCA-psbM_p3	CGATATGGCCAAGCGGTAAG	GCTAGTAGTCAGGGCGGTTA	55.0	55.0	58.9	58.6	1576 bp	1.0	69.3
trnC-GCA-psbM_p4	AGTTCAAATCTGGGTGTCGC	AAACAGCTAGTAGTCAGGGC	50.0	50.0	58.5	56.9	1534 bp	1.0	68.7
trnC-GCA-psbM_p5	GATATGGCCAAGCGGTAAGG	AAACAGCTAGTAGTCAGGGC	55.0	50.0	58.5	56.9	1580 bp	1.0	68.7