Hotspot

atpF-atpH

Genome · 66953-67259 · 307 bp

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Pi 0.0163

MarkerSeek score 62.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: Genome
length_bp: 307
variable_sites: 5
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCGAAATTACCAGCAATAGACC	AAGGTAAAATCCGAGGCACA	45.5	45.0	57.2	57.1	479 bp	1.0	51.4
atpF-atpH_p2	CCGAAATTACCAGCAATAGACC	GCTTTCATCCGCAAATCCTT	45.5	45.0	57.2	57.1	397 bp	1.0	50.9
atpF-atpH_p3	CCGAAATTACCAGCAATAGACC	AGAAGGTAAAATCCGAGGCAC	45.5	47.6	57.2	58.0	481 bp	1.0	50.2
atpF-atpH_p4	CCGAAATTACCAGCAATAGACC	CAGAAGGTAAAATCCGAGGCA	45.5	47.6	57.2	58.0	482 bp	1.0	50.2
atpF-atpH_p5	CCGAAATTACCAGCAATAGACC	AGGTAAAATCCGAGGCACAT	45.5	45.0	57.2	56.9	478 bp	1.0	49.5