Hotspot

psbD-trnT-GGU

Genome · 45809-46326 · 518 bp

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Pi 0.0155

MarkerSeek score 51.40

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 518
variable_sites: 8
pi: 0.0155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AGGCAAAAGCACCATGAAGA	GTCGCCTCCTTGAAAGAGAG	45.0	55.0	58.0	58.0	1266-1267 bp	1.0	80.8
psbD-trnT-GGU_p2	AGGGAGAAATAAGCGCATGG	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.0	1036-1037 bp	1.0	80.8
psbD-trnT-GGU_p3	GGGAGAAATAAGCGCATGGA	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.0	1035-1036 bp	1.0	80.7
psbD-trnT-GGU_p4	CCAACCTGATTGACCGAGAG	GTCGCCTCCTTGAAAGAGAG	55.0	55.0	58.0	58.0	1403-1404 bp	1.0	80.6
psbD-trnT-GGU_p5	GTGCCAAACTATTAGCGGGA	GTCGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.0	1161-1162 bp	1.0	80.6