Hotspot

psbZ-trnS-UGA

Genome · 42953-43174 · 222 bp

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Pi 0.0225

MarkerSeek score 56.00

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: Genome
length_bp: 222
variable_sites: 5
pi: 0.0225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CCAACTGGAAGGCAATAGTCA	CATAAACCACTCGGCCATCT	47.6	50.0	57.9	57.7	268-269 bp	1.0	70.3
psbZ-trnS-UGA_p2	CCAACTGGAAGGCAATAGTCA	TCAAGACCGGAGCCATAAAC	47.6	50.0	57.9	57.6	281-282 bp	1.0	69.7
psbZ-trnS-UGA_p3	CCAACTGGAAGGCAATAGTCA	TAAACCACTCGGCCATCTCT	47.6	50.0	57.9	58.4	266-267 bp	1.0	69.5
psbZ-trnS-UGA_p4	CCAACTGGAAGGCAATAGTCA	GTTTTCAAGACCGGAGCCAT	47.6	50.0	57.9	58.5	285-286 bp	1.0	69.1
psbZ-trnS-UGA_p5	CCAACTGGAAGGCAATAGTCA	CAAGACCGGAGCCATAAACC	47.6	55.0	57.9	58.6	280-281 bp	1.0	67.9