Hotspot

rbcL-atpB

Genome · 27387-27937 · 551 bp

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Pi 0.00376

MarkerSeek score 63.20

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 551
variable_sites: 2
pi: 0.00376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	GTCTCCGTTTGTGGTGACAT	AGCAATGAGTGTGGAAGTCC	50.0	50.0	58.1	57.8	598-616 bp	1.0	80.3
rbcL-atpB_p2	GTCTCCGTTTGTGGTGACAT	AGGCATTTTACCTGGGGAGA	50.0	50.0	58.1	58.3	670-688 bp	1.0	79.0
rbcL-atpB_p3	AACACCTGCTTTAGTCTCCG	AGCAATGAGTGTGGAAGTCC	50.0	50.0	57.5	57.8	611-629 bp	1.0	77.5
rbcL-atpB_p4	CACCTGCTTTAGTCTCCGTT	AGCAATGAGTGTGGAAGTCC	50.0	50.0	57.5	57.8	609-627 bp	1.0	77.5
rbcL-atpB_p5	GTCTCCGTTTGTGGTGACAT	GGCATTTTACCTGGGGAGAA	50.0	50.0	58.1	57.5	669-687 bp	1.0	77.3