Hotspot

psbJ-petA

Genome · 19389-20492 · 1104 bp

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Pi 0.00453

MarkerSeek score 61.40

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 1104
variable_sites: 5
pi: 0.00453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	GCAAATACCACCGGAAGAAT	GCACAAATATTTTCGGTACCCA	45.0	40.9	56.1	57.3	1293-1300 bp	1.0	43.4
psbJ-petA_p2	GGCTCATACTCTGGATTAGGG	GCACAAATATTTTCGGTACCCA	52.4	40.9	57.0	57.3	1206-1213 bp	1.0	42.9
psbJ-petA_p3	GCAAATACCACCGGAAGAATTC	GCACAAATATTTTCGGTACCCA	45.5	40.9	58.0	57.3	1293-1300 bp	1.0	42.4
psbJ-petA_p4	GCAAATACCACCGGAAGAAT	TCGGTACCCAAAAAGAAACA	45.0	40.0	56.1	55.1	1281-1288 bp	1.0	41.9
psbJ-petA_p5	GCAAATACCACCGGAAGAATT	GCACAAATATTTTCGGTACCCA	42.9	40.9	56.9	57.3	1293-1300 bp	1.0	41.5