Hotspot

psbN-psbT

Genome · 10575-10656 · 82 bp

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Pi 0.0732

MarkerSeek score 84.10

Primer available yes

Label psbN-psbT

Feature Metadata

label_name: psbN-psbT
feature_type: igs
region: Genome
length_bp: 82
variable_sites: 6
pi: 0.0732
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbT_p1	CAGCTTTTGGACAACCCTCT	TCGTGAACCTCCCAAAATTCA	50.0	42.9	58.0	57.8	181 bp	1.0	72.8
psbN-psbT_p2	ACAGCTTTTGGACAACCCTC	TCGTGAACCTCCCAAAATTCA	50.0	42.9	58.3	57.8	182 bp	1.0	70.4
psbN-psbT_p3	CAGCTTTTGGACAACCCTCT	CGTGAACCTCCCAAAATTCA	50.0	45.0	58.0	56.2	180 bp	1.0	68.5
psbN-psbT_p4	CAGCTTTTGGACAACCCTCT	CGTGAACCTCCCAAAATTCAA	50.0	42.9	58.0	57.0	180 bp	1.0	66.6
psbN-psbT_p5	CAGCTTTTGGACAACCCTCT	TCGTGAACCTCCCAAAATTC	50.0	45.0	58.0	56.0	181 bp	1.0	66.2