primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	rps3	1	AGCCCAAGGACGAGGATATC	GGTCGCACACTAAGCATAAC	20	20	55.0	50.0	58.7	56.9	0.0	0.0	1.744341	2881	3537	890-890	1.000000	66.4
rps3_p2	rps3	2	GAGCCCAAGGACGAGGATAT	GGTCGCACACTAAGCATAAC	20	20	55.0	50.0	58.7	56.9	0.0	0.0	1.744341	2881	3537	891-891	1.000000	66.4
rps3_p3	rps3	3	GAGCCCAAGGACGAGGATA	GGTCGCACACTAAGCATAAC	19	20	57.9	50.0	58.2	56.9	0.0	0.0	2.265195	2881	3537	891-891	1.000000	62.2
rps3_p4	rps3	4	AGCCCAAGGACGAGGATATC	GGTCGCACACTAAGCATAACA	20	21	55.0	47.6	58.7	58.7	0.0	0.0	2.313830	2881	3537	890-890	1.000000	61.8
rps3_p5	rps3	5	GAGCCCAAGGACGAGGATAT	GGTCGCACACTAAGCATAACA	20	21	55.0	47.6	58.7	58.7	0.0	0.0	2.313830	2881	3537	891-891	1.000000	61.8
petB_p1	petB	1	TGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCA	25	22	28.0	36.4	54.8	55.5	0.0	0.0	12.684111	8761	9976	1286-1292	1.000000	40.4
petB_p2	petB	2	TGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCAA	25	23	28.0	34.8	54.8	56.2	0.0	0.0	12.959274	8761	9976	1286-1292	1.000000	40.4
petB_p3	petB	3	TTGTTAATACCTTCATTCAAATAGCT	TGGTAGTTTAATCGTGTAAGCA	26	22	26.9	36.4	55.5	55.5	0.0	0.0	13.004211	8761	9976	1287-1293	1.000000	40.4
petB_p4	petB	4	TGTTAATACCTTCATTCAAATAGC	TGGTAGTTTAATCGTGTAAGCA	24	22	29.2	36.4	53.4	55.5	0.0	0.0	13.056093	8761	9976	1286-1292	1.000000	40.4
petB_p5	petB	5	TGTTAATACCTTCATTCAAATAGCT	ATGGTAGTTTAATCGTGTAAGCA	25	23	28.0	34.8	54.8	56.0	0.0	0.0	13.161573	8761	9976	1287-1293	1.000000	40.4
psbN-psbT_p1	psbN-psbT	1	CAGCTTTTGGACAACCCTCT	TCGTGAACCTCCCAAAATTCA	20	21	50.0	42.9	58.0	57.8	0.0	0.0	1.229305	10575	10656	181-181	1.000000	72.8
psbN-psbT_p2	psbN-psbT	2	ACAGCTTTTGGACAACCCTC	TCGTGAACCTCCCAAAATTCA	20	21	50.0	42.9	58.3	57.8	0.0	0.0	1.523777	10575	10656	182-182	1.000000	70.4
psbN-psbT_p3	psbN-psbT	3	CAGCTTTTGGACAACCCTCT	CGTGAACCTCCCAAAATTCA	20	20	50.0	45.0	58.0	56.2	0.0	0.0	1.765687	10575	10656	180-180	1.000000	68.5
psbN-psbT_p4	psbN-psbT	4	CAGCTTTTGGACAACCCTCT	CGTGAACCTCCCAAAATTCAA	20	21	50.0	42.9	58.0	57.0	0.0	0.0	2.013168	10575	10656	180-180	1.000000	66.6
psbN-psbT_p5	psbN-psbT	5	CAGCTTTTGGACAACCCTCT	TCGTGAACCTCCCAAAATTC	20	20	50.0	45.0	58.0	56.0	0.0	0.0	2.056154	10575	10656	181-181	1.000000	66.2
rps12-rpl20_p1	rps12-rpl20	1	CTCAACGACGAGGAGTTTGT	CCTCGAAACCCCGATGTAAG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.199732	14746	15569	932-933	1.000000	78.7
rps12-rpl20_p2	rps12-rpl20	2	CTCAACGACGAGGAGTTTGT	CTCGAAACCCCGATGTAAGG	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.199732	14746	15569	931-932	1.000000	78.7
rps12-rpl20_p3	rps12-rpl20	3	ACAAAATCTCCAGCCCTTCG	CCTCGAAACCCCGATGTAAG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.237683	14746	15569	960-961	1.000000	78.4
rps12-rpl20_p4	rps12-rpl20	4	ACAAAATCTCCAGCCCTTCG	CTCGAAACCCCGATGTAAGG	20	20	50.0	55.0	58.2	58.1	0.0	0.0	0.237683	14746	15569	959-960	1.000000	78.4
rps12-rpl20_p5	rps12-rpl20	5	CTCAACGACGAGGAGTTTGT	AATTTCGAATGAGCCCCTCG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.467245	14746	15569	947-948	1.000000	76.6
psbJ-petA_p1	psbJ-petA	1	GCAAATACCACCGGAAGAAT	GCACAAATATTTTCGGTACCCA	20	22	45.0	40.9	56.1	57.3	9.5	6.3	4.609639	19389	20492	1293-1300	1.000000	43.4
psbJ-petA_p2	psbJ-petA	2	GGCTCATACTCTGGATTAGGG	GCACAAATATTTTCGGTACCCA	21	22	52.4	40.9	57.0	57.3	0.0	6.3	4.676582	19389	20492	1206-1213	1.000000	42.9
psbJ-petA_p3	psbJ-petA	3	GCAAATACCACCGGAAGAATTC	GCACAAATATTTTCGGTACCCA	22	22	45.5	40.9	58.0	57.3	9.5	6.3	4.734189	19389	20492	1293-1300	1.000000	42.4
psbJ-petA_p4	psbJ-petA	4	GCAAATACCACCGGAAGAAT	TCGGTACCCAAAAAGAAACA	20	20	45.0	40.0	56.1	55.1	9.5	6.3	4.797718	19389	20492	1281-1288	1.000000	41.9
psbJ-petA_p5	psbJ-petA	5	GCAAATACCACCGGAAGAATT	GCACAAATATTTTCGGTACCCA	21	22	42.9	40.9	56.9	57.3	9.5	6.3	4.848472	19389	20492	1293-1300	1.000000	41.5
rbcL-atpB_p1	rbcL-atpB	1	GTCTCCGTTTGTGGTGACAT	AGCAATGAGTGTGGAAGTCC	20	20	50.0	50.0	58.1	57.8	9.4	0.0	0.312175	27387	27937	598-616	1.000000	80.3
rbcL-atpB_p2	rbcL-atpB	2	GTCTCCGTTTGTGGTGACAT	AGGCATTTTACCTGGGGAGA	20	20	50.0	50.0	58.1	58.3	9.4	0.0	0.439818	27387	27937	670-688	1.000000	79.0
rbcL-atpB_p3	rbcL-atpB	3	AACACCTGCTTTAGTCTCCG	AGCAATGAGTGTGGAAGTCC	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.648494	27387	27937	611-629	1.000000	77.5
rbcL-atpB_p4	rbcL-atpB	4	CACCTGCTTTAGTCTCCGTT	AGCAATGAGTGTGGAAGTCC	20	20	50.0	50.0	57.5	57.8	0.0	0.0	0.648494	27387	27937	609-627	1.000000	77.5
rbcL-atpB_p5	rbcL-atpB	5	GTCTCCGTTTGTGGTGACAT	GGCATTTTACCTGGGGAGAA	20	20	50.0	50.0	58.1	57.5	9.4	0.0	0.646152	27387	27937	669-687	1.000000	77.3
psbZ-trnS-UGA_p1	psbZ-trnS-UGA	1	CCAACTGGAAGGCAATAGTCA	CATAAACCACTCGGCCATCT	21	20	47.6	50.0	57.9	57.7	0.0	2.2	1.403937	42953	43174	268-269	1.000000	70.3
psbZ-trnS-UGA_p2	psbZ-trnS-UGA	2	CCAACTGGAAGGCAATAGTCA	TCAAGACCGGAGCCATAAAC	21	20	47.6	50.0	57.9	57.6	0.0	9.5	1.468463	42953	43174	281-282	1.000000	69.7
psbZ-trnS-UGA_p3	psbZ-trnS-UGA	3	CCAACTGGAAGGCAATAGTCA	TAAACCACTCGGCCATCTCT	21	20	47.6	50.0	57.9	58.4	0.0	2.2	1.503370	42953	43174	266-267	1.000000	69.5
psbZ-trnS-UGA_p4	psbZ-trnS-UGA	4	CCAACTGGAAGGCAATAGTCA	GTTTTCAAGACCGGAGCCAT	21	20	47.6	50.0	57.9	58.5	0.0	9.5	1.537145	42953	43174	285-286	1.000000	69.1
psbZ-trnS-UGA_p5	psbZ-trnS-UGA	5	CCAACTGGAAGGCAATAGTCA	CAAGACCGGAGCCATAAACC	21	20	47.6	55.0	57.9	58.6	0.0	9.5	1.692347	42953	43174	280-281	1.000000	67.9
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	AGGCAAAAGCACCATGAAGA	GTCGCCTCCTTGAAAGAGAG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.003548	45809	46326	1266-1267	1.000000	80.8
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	AGGGAGAAATAAGCGCATGG	GTCGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	58.0	58.0	11.5	0.0	0.030324	45809	46326	1036-1037	1.000000	80.8
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	GGGAGAAATAAGCGCATGGA	GTCGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	58.0	58.0	11.5	0.0	0.035878	45809	46326	1035-1036	1.000000	80.7
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	CCAACCTGATTGACCGAGAG	GTCGCCTCCTTGAAAGAGAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.018932	45809	46326	1403-1404	1.000000	80.6
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	GTGCCAAACTATTAGCGGGA	GTCGCCTCCTTGAAAGAGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.035877	45809	46326	1161-1162	1.000000	80.6
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	AACCTCTGTCAATGGCTTGG	TACGGAAAAATTTGCACGCC	20	20	50.0	45.0	58.1	57.9	0.0	8.1	0.138141	58528	59424	1084-1085	1.000000	79.6
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	AACCTCTGTCAATGGCTTGG	TGTATGCAGCCAAAGAAGCT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.289881	58528	59424	1031-1032	1.000000	78.5
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	AGTTTCGCAAACCTCTGTCA	TACGGAAAAATTTGCACGCC	20	20	45.0	45.0	57.7	57.9	7.8	8.1	0.378865	58528	59424	1093-1094	1.000000	77.7
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	CCTCTGTCAATGGCTTGGAT	TACGGAAAAATTTGCACGCC	20	20	50.0	45.0	57.6	57.9	0.0	8.1	0.485236	58528	59424	1082-1083	1.000000	76.9
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	AACCTCTGTCAATGGCTTGG	AAATTTGCACGCCGAAATGA	20	20	50.0	40.0	58.1	57.6	0.0	0.0	0.515405	58528	59424	1077-1078	1.000000	76.6
trnG-UCC_p1	trnG-UCC	1	CTGGCCAGGTTACCCTTTTT	ATTTGCACAATTCGCATCCG	20	20	50.0	45.0	58.0	58.1	26.1	0.1	0.097680	63239	63889	1887-1887	1.000000	79.9
trnG-UCC_p2	trnG-UCC	2	CTGGCCAGGTTACCCTTTTT	TTCGCCCTGCAATCAATGTA	20	20	50.0	45.0	58.0	57.9	26.1	0.0	0.130973	63239	63889	2011-2011	1.000000	79.7
trnG-UCC_p3	trnG-UCC	3	CTGGCCAGGTTACCCTTTTT	TAGGTTCTGCGGCTCAAATC	20	20	50.0	50.0	58.0	58.0	26.1	0.0	0.033857	63239	63889	1972-1972	0.500000	59.7
trnG-UCC_p4	trnG-UCC	4	GCAGCACGTAAAGGCAATTT	TAGGTTCTGCGGCTCAAATC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.088749	63239	63889	1507-1507	0.500000	59.3
trnG-UCC_p5	trnG-UCC	5	TAGCAGCACGTAAAGGCAAT	TAGGTTCTGCGGCTCAAATC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.132126	63239	63889	1509-1509	0.500000	58.9
atpF-atpH_p1	atpF-atpH	1	CCGAAATTACCAGCAATAGACC	AAGGTAAAATCCGAGGCACA	22	20	45.5	45.0	57.2	57.1	0.0	0.0	3.684393	66953	67259	479-479	1.000000	51.4
atpF-atpH_p2	atpF-atpH	2	CCGAAATTACCAGCAATAGACC	GCTTTCATCCGCAAATCCTT	22	20	45.5	45.0	57.2	57.1	0.0	0.0	3.760744	66953	67259	397-397	1.000000	50.9
atpF-atpH_p3	atpF-atpH	3	CCGAAATTACCAGCAATAGACC	AGAAGGTAAAATCCGAGGCAC	22	21	45.5	47.6	57.2	58.0	0.0	0.0	3.826064	66953	67259	481-481	1.000000	50.2
atpF-atpH_p4	atpF-atpH	4	CCGAAATTACCAGCAATAGACC	CAGAAGGTAAAATCCGAGGCA	22	21	45.5	47.6	57.2	58.0	0.0	0.0	3.827401	66953	67259	482-482	1.000000	50.2
atpF-atpH_p5	atpF-atpH	5	CCGAAATTACCAGCAATAGACC	AGGTAAAATCCGAGGCACAT	22	20	45.5	45.0	57.2	56.9	0.0	0.0	3.915138	66953	67259	478-478	1.000000	49.5
trnC-GCA-psbM_p1	trnC-GCA-psbM	1	AGTTCAAATCTGGGTGTCGC	GCTAGTAGTCAGGGCGGTTA	20	20	50.0	55.0	58.5	58.6	0.0	0.0	1.079590	80265	81750	1529-1529	1.000000	72.4
trnC-GCA-psbM_p2	trnC-GCA-psbM	2	GATATGGCCAAGCGGTAAGG	GCTAGTAGTCAGGGCGGTTA	20	20	55.0	55.0	58.5	58.6	11.0	0.0	1.079590	80265	81750	1575-1575	1.000000	72.3
trnC-GCA-psbM_p3	trnC-GCA-psbM	3	CGATATGGCCAAGCGGTAAG	GCTAGTAGTCAGGGCGGTTA	20	20	55.0	55.0	58.9	58.6	11.0	0.0	1.455038	80265	81750	1576-1576	1.000000	69.3
trnC-GCA-psbM_p4	trnC-GCA-psbM	4	AGTTCAAATCTGGGTGTCGC	AAACAGCTAGTAGTCAGGGC	20	20	50.0	50.0	58.5	56.9	0.0	0.0	1.537632	80265	81750	1534-1534	1.000000	68.7
trnC-GCA-psbM_p5	trnC-GCA-psbM	5	GATATGGCCAAGCGGTAAGG	AAACAGCTAGTAGTCAGGGC	20	20	55.0	50.0	58.5	56.9	11.0	0.0	1.537632	80265	81750	1580-1580	1.000000	68.7
psbM-trnL-CAA_p1	psbM-trnL-CAA	1	TTAGTGAAAAGGCTGTGGGT	AGGCCTTGATGGTGAAATGG	20	20	45.0	50.0	57.0	58.1	0.0	0.0	1.185711	81859	82084	379-379	1.000000	70.5
psbM-trnL-CAA_p2	psbM-trnL-CAA	2	AGTGAAAAGGCTGTGGGTAT	AGGCCTTGATGGTGAAATGG	20	20	45.0	50.0	56.7	58.1	0.0	0.0	1.419018	81859	82084	377-377	1.000000	68.6
psbM-trnL-CAA_p3	psbM-trnL-CAA	3	TTAGTGAAAAGGCTGTGGGT	AACAGGCCTTGATGGTGAAA	20	20	45.0	45.0	57.0	57.6	0.0	0.0	1.422404	81859	82084	382-382	1.000000	68.6
psbM-trnL-CAA_p4	psbM-trnL-CAA	4	TTAGTGAAAAGGCTGTGGGT	GGCCTTGATGGTGAAATGGT	20	20	45.0	50.0	57.0	58.4	0.0	0.0	1.481835	81859	82084	378-378	1.000000	68.1
psbM-trnL-CAA_p5	psbM-trnL-CAA	5	TTAGTGAAAAGGCTGTGGGT	TTAAAGCGTGGAGGTTCGAA	20	20	45.0	45.0	57.0	57.5	0.0	0.0	1.572120	81859	82084	330-330	1.000000	67.4
trnL-CAA-ndhB_p1	trnL-CAA-ndhB	1	TCGAACCTCCACGCTTTAAA	ACTTGTGTAATTGCTTCTATGT	20	22	45.0	31.8	57.5	53.7	0.0	0.0	6.784720	82166	82358	353-358	1.000000	42.0
trnL-CAA-ndhB_p2	trnL-CAA-ndhB	2	TTCGAACCTCCACGCTTTAA	ACTTGTGTAATTGCTTCTATGT	20	22	45.0	31.8	57.5	53.7	0.0	0.0	6.784720	82166	82358	354-359	1.000000	42.0
trnL-CAA-ndhB_p3	trnL-CAA-ndhB	3	GATTCGAACCTCCACGCTTT	ACTTGTGTAATTGCTTCTATGT	20	22	50.0	31.8	58.6	53.7	0.0	0.0	6.815260	82166	82358	356-361	1.000000	42.0
trnL-CAA-ndhB_p4	trnL-CAA-ndhB	4	TCGAACCTCCACGCTTTAAA	ACTTGTGTAATTGCTTCTATGTT	20	23	45.0	30.4	57.5	54.6	0.0	0.0	6.977648	82166	82358	353-358	1.000000	42.0
trnL-CAA-ndhB_p5	trnL-CAA-ndhB	5	TTCGAACCTCCACGCTTTAA	ACTTGTGTAATTGCTTCTATGTT	20	23	45.0	30.4	57.5	54.6	0.0	0.0	6.977648	82166	82358	354-359	1.000000	42.0
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	GCCACTTTACGTAAGGCAGA	GGCTCGAACTGATGACTTCC	20	20	50.0	55.0	57.9	58.4	24.5	0.0	0.442760	85942	87316	1478-1479	1.000000	76.8
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	GCCACTTTACGTAAGGCAGA	GCTCGAACTGATGACTTCCA	20	20	50.0	50.0	57.9	57.6	24.5	0.0	0.459231	85942	87316	1477-1478	1.000000	76.7
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GCCACTTTACGTAAGGCAGA	CAGGCTCGAACTGATGACTT	20	20	50.0	50.0	57.9	57.6	24.5	10.4	0.465757	85942	87316	1480-1481	1.000000	76.6
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GCCACTTTACGTAAGGCAGA	TCAGGCTCGAACTGATGACT	20	20	50.0	50.0	57.9	58.4	24.5	13.7	0.538623	85942	87316	1481-1482	1.000000	76.0
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	GCCACTTTACGTAAGGCAGA	CGAACTGATGACTTCCACCA	20	20	50.0	50.0	57.9	57.5	24.5	0.0	0.544623	85942	87316	1474-1475	1.000000	76.0
ndhH_p1	ndhH	1	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTGA	20	23	35.0	39.1	53.8	56.9	0.0	0.0	8.374017	103651	104826	1326-1326	1.000000	40.2
ndhH_p2	ndhH	2	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTGAA	20	24	35.0	37.5	53.8	57.5	0.0	0.0	8.736009	103651	104826	1326-1326	1.000000	40.2
ndhH_p3	ndhH	3	AATGAATGAAAAGGAGCGTT	GCTAACAAGAGAATGGTGATTG	20	22	35.0	40.9	53.8	55.4	0.0	0.0	8.790602	103651	104826	1326-1326	1.000000	40.2
ndhH_p4	ndhH	4	ATGAATGAAAAGGAGCGTTA	GCTAACAAGAGAATGGTGATTGA	20	23	35.0	39.1	52.9	56.9	0.0	0.0	9.253072	103651	104826	1325-1325	1.000000	40.2
ndhH_p5	ndhH	5	TGAATGAAAAGGAGCGTTAT	GCTAACAAGAGAATGGTGATTGA	20	23	35.0	39.1	52.9	56.9	0.0	0.0	9.253072	103651	104826	1324-1324	1.000000	40.2