Hotspot

rpl14

IRa · 163963-164331 · 369 bp

Back to Dicranostigma

Pi 0.438

MarkerSeek score 62.90

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: IRa
length_bp: 369
variable_sites: 28
pi: 0.438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.17
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	TTGCATTGAAAGAAACGATT	ATTGTATCGTGGTCTCGTCT	30.0	45.0	52.2	56.1	427 bp	0.5	20.0
rpl14_p2	TTTGCATTGAAAGAAACGAT	ATTGTATCGTGGTCTCGTCT	30.0	45.0	52.2	56.1	428 bp	0.5	20.0
rpl14_p3	TTTGCATTGAAAGAAACGATT	ATTGTATCGTGGTCTCGTCT	28.6	45.0	53.2	56.1	428 bp	0.5	20.0
rpl14_p4	TTGCATTGAAAGAAACGATT	TTGTATCGTGGTCTCGTCTA	30.0	45.0	52.2	55.5	426 bp	0.5	20.0
rpl14_p5	TTTGCATTGAAAGAAACGAT	TTGTATCGTGGTCTCGTCTA	30.0	45.0	52.2	55.5	427 bp	0.5	20.0