Hotspot

atpE

LSC · 57013-57414 · 402 bp

Back to Dicranostigma

Pi 0.51

MarkerSeek score 77.00

Primer available yes

Label atpE

Feature Metadata

label_name: atpE
feature_type: gene
region: LSC
length_bp: 402
variable_sites: 177
pi: 0.51
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpE_p1	TTGGTTTGACTGCCCTAACC	TGGGTTCTATTTGGGGCCTA	50.0	50.0	58.0	58.0	1803 bp	0.5	59.8
atpE_p2	GCAGTTTATGTACCTGCGGA	TGGGTTCTATTTGGGGCCTA	50.0	50.0	58.0	58.0	1573 bp	0.5	59.8
atpE_p3	TTGGTTTGACTGCCCTAACC	TATTATTTGGACGCACCGCA	50.0	45.0	58.0	58.0	1178 bp	0.5	59.7
atpE_p4	TTGGTTTGACTGCCCTAACC	TGGGCTGGGTATTCACCTTA	50.0	50.0	58.0	58.0	1842 bp	0.5	59.7
atpE_p5	GCAGTTTATGTACCTGCGGA	TATTATTTGGACGCACCGCA	50.0	45.0	58.0	58.0	948 bp	0.5	59.6