Hotspot

rps15-trnN-GUU

SSC · 129156-135066 · 5911 bp

Back to Dicranostigma

Pi 0.119

MarkerSeek score 77.70

Primer available yes

Label rps15-trnN-GUU

Feature Metadata

label_name: rps15-trnN-GUU
feature_type: igs
region: SSC
length_bp: 5911
variable_sites: 651
pi: 0.119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-trnN-GUU_p1	TGACTCTTATGGGCCAGTGA	GACCTGGACCGAGATGGATA	50.0	55.0	58.0	58.0	798 bp	0.5	59.6
rps15-trnN-GUU_p2	GCGTGTGTACATCTGTTTGC	GACCTGGACCGAGATGGATA	50.0	55.0	58.0	58.0	1212 bp	0.5	59.6
rps15-trnN-GUU_p3	AAATTGGCCGCAGTTCCTTA	GACCTGGACCGAGATGGATA	45.0	55.0	58.1	58.0	985 bp	0.5	59.3
rps15-trnN-GUU_p4	TAAATTGGCCGCAGTTCCTT	GACCTGGACCGAGATGGATA	45.0	55.0	58.1	58.0	986 bp	0.5	59.3
rps15-trnN-GUU_p5	TCTGTAAATTGGCCGCAGTT	GACCTGGACCGAGATGGATA	45.0	55.0	58.1	58.0	990 bp	0.5	59.2