Hotspot

ccsA-ndhD

SSC · 120984-121187 · 204 bp

Back to Dicranostigma

Pi 0.0792

MarkerSeek score 80.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 204
variable_sites: 16
pi: 0.0792
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTGGCTTCTA	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.8	57.4	389-436 bp	1.0	76.8
ccsA-ndhD_p2	TTCCGCAATTGTGGCTTCTA	CCGGATTTCGTTCTCTCACT	45.0	50.0	57.8	57.3	388-435 bp	1.0	76.7
ccsA-ndhD_p3	AATTCCGCAATTGTGGCTTC	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.6	57.4	391-438 bp	1.0	75.3
ccsA-ndhD_p4	AATTCCGCAATTGTGGCTTC	CCGGATTTCGTTCTCTCACT	45.0	50.0	57.6	57.3	390-437 bp	1.0	75.3
ccsA-ndhD_p5	TCCGCAATTGTGGCTTCTAT	TCCGGATTTCGTTCTCTCAC	45.0	50.0	57.6	57.4	388-435 bp	1.0	75.0