Hotspot

trnN-GUU-ndhF

IRb · 114681-115250 · 570 bp

Back to Dicranostigma

Pi 0.158

MarkerSeek score 82.40

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 570
variable_sites: 86
pi: 0.158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AACTCTTCCTCAGTCAGGCT	ATTTCCCCATACCCCCATGA	50.0	50.0	58.0	58.1	1649 bp	0.5	59.3
trnN-GUU-ndhF_p2	AACTCTTCCTCAGTCAGGCT	TTCCCCATACCCCCATGAAT	50.0	50.0	58.0	58.1	1647 bp	0.5	59.3
trnN-GUU-ndhF_p3	ACTCTTCCTCAGTCAGGCTT	ATTTCCCCATACCCCCATGA	50.0	50.0	58.0	58.1	1648 bp	0.5	59.3
trnN-GUU-ndhF_p4	ACTCTTCCTCAGTCAGGCTT	TTCCCCATACCCCCATGAAT	50.0	50.0	58.0	58.1	1646 bp	0.5	59.3
trnN-GUU-ndhF_p5	ACTCTTCCTCAGTCAGGCTT	AGTCTTGGTTCGACTCATGC	50.0	50.0	58.0	57.9	1217 bp	0.5	59.1