Hotspot

rpl16-rps3

IRb · 87337-88108 · 772 bp

Back to Dicranostigma

Pi 0.425

MarkerSeek score 80.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: IRb
length_bp: 772
variable_sites: 252
pi: 0.425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GACGAGTCACACACTAAGCA	TTGGATATTTGCGGACGAGG	50.0	50.0	57.6	58.1	732-818 bp	1.0	88.2
rpl16-rps3_p2	GACGAGTCACACACTAAGCA	GGATATTTGCGGACGAGGAA	50.0	50.0	57.6	57.8	730-816 bp	1.0	86.8
rpl16-rps3_p3	GACGAGTCACACACTAAGCAT	TTGGATATTTGCGGACGAGG	47.6	50.0	58.1	58.1	732-818 bp	1.0	83.2
rpl16-rps3_p4	ACGAGTCACACACTAAGCAT	TTGGATATTTGCGGACGAGG	45.0	50.0	56.9	58.1	731-817 bp	1.0	82.7
rpl16-rps3_p5	GACGAGTCACACACTAAGCAT	GGATATTTGCGGACGAGGAA	47.6	50.0	58.1	57.8	730-816 bp	1.0	81.7