Hotspot

petA-psbJ

LSC · 67174-68282 · 1109 bp

Back to Dicranostigma

Pi 0.055

MarkerSeek score 86.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1109
variable_sites: 59
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	GATCGGTTCAGTCGGTGTTT	50.0	50.0	57.8	58.2	1242-1272 bp	1.0	83.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	ATCGGTTCAGTCGGTGTTTT	50.0	45.0	57.8	57.8	1241-1271 bp	1.0	82.9
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1207-1237 bp	1.0	82.1
petA-psbJ_p4	GATCCATCACGTGTCCAAGG	GATCGGTTCAGTCGGTGTTT	55.0	50.0	58.3	58.2	1260-1290 bp	1.0	81.6
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	CGGTTCAGTCGGTGTTTTCT	50.0	50.0	57.8	58.4	1239-1269 bp	1.0	81.5