Hotspot

rbcL-psaI

LSC · 60236-63270 · 3035 bp

Back to Dicranostigma

Pi 0.318

MarkerSeek score 78.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 3035
variable_sites: 696
pi: 0.318
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TTGTCCCTTTCGTCCTCTCT	CAAACGGGTGATTTTCTCGC	50.0	50.0	58.0	58.0	1312 bp	0.5	59.6
rbcL-psaI_p2	CTGCGGTCTCTGTTGTAGTC	CAAACGGGTGATTTTCTCGC	55.0	50.0	58.0	58.0	1231 bp	0.5	59.6
rbcL-psaI_p3	TTGTCCCTTTCGTCCTCTCT	GGATTCCAAGTGTCCGGATC	50.0	55.0	58.0	58.0	1089 bp	0.5	59.6
rbcL-psaI_p4	CTGCGGTCTCTGTTGTAGTC	GGATTCCAAGTGTCCGGATC	55.0	55.0	58.0	58.0	1008 bp	0.5	59.5
rbcL-psaI_p5	TTGTCCCTTTCGTCCTCTCT	TGATTTTCTCGCCGACTACG	50.0	50.0	58.0	58.1	1304 bp	0.5	59.1