Hotspot

trnM-CAU-rbcL

LSC · 57688-58807 · 1120 bp

Back to Dicranostigma

Pi 0.355

MarkerSeek score 79.30

Primer available yes

Label trnM-CAU-rbcL

Feature Metadata

label_name: trnM-CAU-rbcL
feature_type: igs
region: LSC
length_bp: 1120
variable_sites: 318
pi: 0.355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-rbcL_p1	TGCGGTGCGTCCAAATAATA	CGGTCTCTCCAACGCATAAA	45.0	50.0	58.0	58.0	2035 bp	0.5	59.8
trnM-CAU-rbcL_p2	TGCGGTGCGTCCAAATAATA	GTAAGTCCATCGGTCCACAC	45.0	55.0	58.0	58.0	1609 bp	0.5	59.7
trnM-CAU-rbcL_p3	AAAATAGCTCTGATGGGCGG	CGGTCTCTCCAACGCATAAA	50.0	50.0	58.0	58.0	2271 bp	0.5	59.7
trnM-CAU-rbcL_p4	AAAATAGCTCTGATGGGCGG	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1845 bp	0.5	59.7
trnM-CAU-rbcL_p5	TATTGGGGTTCTGCCGATTG	CGGTCTCTCCAACGCATAAA	50.0	50.0	57.9	58.0	1989 bp	0.5	59.6