Hotspot

ndhC-atpB

LSC · 53398-55528 · 2131 bp

Back to Dicranostigma

Pi 0.111

MarkerSeek score 79.50

Primer available yes

Label ndhC-atpB

Feature Metadata

label_name: ndhC-atpB
feature_type: igs
region: LSC
length_bp: 2131
variable_sites: 232
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-atpB_p1	AGTACCGGAAACTCAGTGGA	TGAATAGGGGATGTTGTGCG	50.0	50.0	58.0	58.0	2968 bp	0.5	59.7
ndhC-atpB_p2	AGTACCGGAAACTCAGTGGA	CATCCAGTACCGGACCAATG	50.0	55.0	58.0	58.0	2667 bp	0.5	59.6
ndhC-atpB_p3	AGTACCGGAAACTCAGTGGA	ATTGTATAAAGGCAGGCGCA	50.0	45.0	58.0	57.9	2994 bp	0.5	59.5
ndhC-atpB_p4	GAAACTCATCGCCCATGGAT	TGAATAGGGGATGTTGTGCG	50.0	50.0	58.0	58.0	2778 bp	0.5	59.5
ndhC-atpB_p5	GAAACTCATCGCCCATGGAT	CATCCAGTACCGGACCAATG	50.0	55.0	58.0	58.0	2477 bp	0.5	59.4