Hotspot

ycf1

SSC · 116196-120680 · 4485 bp

Back to Dicranopteris

Pi 0.487

MarkerSeek score 75.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4485
variable_sites: 1838
pi: 0.487
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGACGAGTATTGGAACACC	CCCCATTCATTCCCGCAATA	55.0	50.0	58.0	58.0	1096 bp	0.5	59.8
ycf1_p2	GGGGACGAGTATTGGAACAC	CCCCATTCATTCCCGCAATA	55.0	50.0	58.0	58.0	1097 bp	0.5	59.8
ycf1_p3	GTGGGGACGAGTATTGGAAC	CCCCATTCATTCCCGCAATA	55.0	50.0	58.0	58.0	1099 bp	0.5	59.8
ycf1_p4	GGGACGAGTATTGGAACACC	AGGCTACTCCCCAAAATCCT	55.0	50.0	58.0	58.0	875 bp	0.5	59.7
ycf1_p5	GGGGACGAGTATTGGAACAC	AGGCTACTCCCCAAAATCCT	55.0	50.0	58.0	58.0	876 bp	0.5	59.7