Hotspot

ndhG

SSC · 125697-126293 · 597 bp

Back to Dicranopteris

Pi 0.46

MarkerSeek score 79.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 597
variable_sites: 218
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AAGTGCAAAGGAAGGTGAGG	CCTACGCGATGAGGATAAGC	50.0	55.0	58.0	58.0	899 bp	0.5	59.8
ndhG_p2	ATCAAATGGCTTTGGGACGT	CCTACGCGATGAGGATAAGC	45.0	55.0	58.1	58.0	1077 bp	0.5	59.3
ndhG_p3	TGTGAGGTATGTGTCCGAGT	CCTACGCGATGAGGATAAGC	50.0	55.0	58.1	58.0	1291 bp	0.5	59.2
ndhG_p4	TGCCCGTCATTGATTGGAAA	CCTACGCGATGAGGATAAGC	45.0	55.0	58.1	58.0	1257 bp	0.5	59.2
ndhG_p5	AAGTGCAAAGGAAGGTGAGG	CCCTGTTCAAGCATGACGTA	50.0	50.0	58.0	57.9	866 bp	0.5	59.1