Hotspot

ndhA

SSC · 123127-124806 · 1680 bp

Back to Dicranopteris

Pi 0.471

MarkerSeek score 80.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1680
variable_sites: 682
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGCCTAAGCAGGAACATCAT	ACTCGGACACATACCTCACA	50.0	50.0	58.0	58.1	2679 bp	0.5	59.0
ndhA_p2	CGCCTAAGCAGGAACATCAT	TTTCCAATCAATGACGGGCA	50.0	45.0	58.0	58.1	2713 bp	0.5	59.0
ndhA_p3	CATGTAAGAGTGGAAGCCCC	ACTCGGACACATACCTCACA	55.0	50.0	58.0	58.1	2662 bp	0.5	59.0
ndhA_p4	ATCGCCAAAACGGTAACTGT	CCTCACCTTCCTTTGCACTT	45.0	50.0	58.1	58.0	2858 bp	0.5	59.0
ndhA_p5	CATGTAAGAGTGGAAGCCCC	TTTCCAATCAATGACGGGCA	55.0	45.0	58.0	58.1	2696 bp	0.5	59.0