Hotspot

chlN

SSC · 114307-115365 · 1059 bp

Back to Dicranopteris

Pi 0.505

MarkerSeek score 80.90

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: SSC
length_bp: 1059
variable_sites: 457
pi: 0.505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	TGCTCTCGGAGTTACGATCT	CCCTGCACCACTCTGTATTC	50.0	55.0	58.0	58.0	1402 bp	0.5	59.4
chlN_p2	TGCTCTCGGAGTTACGATCT	GCCAATACGGGACTTGGTAG	50.0	55.0	58.0	58.1	1913 bp	0.5	59.3
chlN_p3	TGCTCTCGGAGTTACGATCT	TACAACGACATTCGAACGCT	50.0	45.0	58.0	57.9	1487 bp	0.5	59.2
chlN_p4	ACTCTCCCCTTTGAGTGTGA	CCCTGCACCACTCTGTATTC	50.0	55.0	57.9	58.0	1539 bp	0.5	58.9
chlN_p5	TGCTCTCGGAGTTACGATCT	CATTACGTGGGGTTTGACCA	50.0	50.0	58.0	58.1	1737 bp	0.5	58.9