Hotspot

chlL

SSC · 113030-113977 · 948 bp

Back to Dicranopteris

Pi 0.497

MarkerSeek score 79.40

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: SSC
length_bp: 948
variable_sites: 378
pi: 0.497
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	TATAGGGGGAAGCGGTAGTG	AGGGATTGACTCCACAGACA	55.0	50.0	58.0	58.0	2110 bp	0.5	59.7
chlL_p2	TATAGGGGGAAGCGGTAGTG	AGATCGTAACTCCGAGAGCA	55.0	50.0	58.0	58.0	1534 bp	0.5	59.6
chlL_p3	AATAGAGGAATCTCCCCGGG	AGGGATTGACTCCACAGACA	55.0	50.0	58.0	58.0	2337 bp	0.5	59.5
chlL_p4	AATAGAGGAATCTCCCCGGG	AGATCGTAACTCCGAGAGCA	55.0	50.0	58.0	58.0	1761 bp	0.5	59.4
chlL_p5	GATAAACTCTTGAGGGGCGG	AGGGATTGACTCCACAGACA	55.0	50.0	58.0	58.0	1852 bp	0.5	59.4