Hotspot

rpl32-trnN-GUU

SSC · 132221-135672 · 3452 bp

Back to Dicranopteris

Pi 0.408

MarkerSeek score 78.40

Primer available yes

Label rpl32-trnN-GUU

Feature Metadata

label_name: rpl32-trnN-GUU
feature_type: igs
region: SSC
length_bp: 3452
variable_sites: 1262
pi: 0.408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnN-GUU_p1	ACTTCCTCGCCAATGTTTGT	AGGGACTTGGAGGAGTGATC	45.0	55.0	58.0	58.1	931 bp	0.5	58.8
rpl32-trnN-GUU_p2	TGCTCTTTCATCATTCCCGG	AGGGACTTGGAGGAGTGATC	50.0	55.0	58.0	58.1	912 bp	0.5	58.6
rpl32-trnN-GUU_p3	ACTTCCTCGCCAATGTTTGT	CACGAGACTCGGTTGTTGAA	45.0	50.0	58.0	58.2	864 bp	0.5	58.6
rpl32-trnN-GUU_p4	ACTTCCTCGCCAATGTTTGT	TCATCTCGTACGAGCGGATA	45.0	50.0	58.0	57.8	835 bp	0.5	58.6
rpl32-trnN-GUU_p5	TGCTCTTTCATCATTCCCGG	CACGAGACTCGGTTGTTGAA	50.0	50.0	58.0	58.2	845 bp	0.5	58.4