Hotspot

trnP-GGG-rpl32

SSC · 130787-132043 · 1257 bp

Back to Dicranopteris

Pi 0.481

MarkerSeek score 80.60

Primer available yes

Label trnP-GGG-rpl32

Feature Metadata

label_name: trnP-GGG-rpl32
feature_type: igs
region: SSC
length_bp: 1257
variable_sites: 510
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-rpl32_p1	TCACCATAGCGGCTTGTTAC	GCTATGGCAGTTCCGAAGAA	50.0	50.0	58.0	58.0	1697 bp	0.5	59.7
trnP-GGG-rpl32_p2	CACCATAGCGGCTTGTTACT	GCTATGGCAGTTCCGAAGAA	50.0	50.0	58.0	58.0	1696 bp	0.5	59.6
trnP-GGG-rpl32_p3	TCACCATAGCGGCTTGTTAC	TGTCTCGGCAAGAAAACCAA	50.0	45.0	58.0	58.0	1730 bp	0.5	59.5
trnP-GGG-rpl32_p4	TCACCATAGCGGCTTGTTAC	TTGTCTCGGCAAGAAAACCA	50.0	45.0	58.0	58.0	1731 bp	0.5	59.5
trnP-GGG-rpl32_p5	CACCATAGCGGCTTGTTACT	TGTCTCGGCAAGAAAACCAA	50.0	45.0	58.0	58.0	1729 bp	0.5	59.4