Hotspot

ccsA-trnL-UAG

SSC · 130199-130459 · 261 bp

Back to Dicranopteris

Pi 0.441

MarkerSeek score 76.10

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 261
variable_sites: 104
pi: 0.441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	GGAGATTTGAGCGAGTGTGT	CCCTACTCGGAATAGCAGGA	50.0	55.0	57.9	58.0	636 bp	0.5	59.3
ccsA-trnL-UAG_p2	GGAGATTTGAGCGAGTGTGT	TCCCCTACTCGGAATAGCAG	50.0	55.0	57.9	58.0	638 bp	0.5	59.3
ccsA-trnL-UAG_p3	GGAGATTTGAGCGAGTGTGT	GTAACAAGCCGCTATGGTGA	50.0	50.0	57.9	58.0	390 bp	0.5	59.2
ccsA-trnL-UAG_p4	GGAGATTTGAGCGAGTGTGT	AGTAACAAGCCGCTATGGTG	50.0	50.0	57.9	58.0	391 bp	0.5	59.1
ccsA-trnL-UAG_p5	AGCGGTGTAGATTGTTGCAT	CCCTACTCGGAATAGCAGGA	45.0	55.0	57.9	58.0	971 bp	0.5	59.1