Hotspot

ndhD-ccsA

SSC · 128951-129232 · 282 bp

Back to Dicranopteris

Pi 0.476

MarkerSeek score 77.30

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 282
variable_sites: 127
pi: 0.476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GCGTAACCTCAAGAGAAGGG	CGAAAGAGACGTGGGCATTA	55.0	50.0	58.0	58.0	1098 bp	0.5	59.9
ndhD-ccsA_p2	TCACGCCCATCCTACATTTG	CGAAAGAGACGTGGGCATTA	50.0	50.0	58.0	58.0	1182 bp	0.5	59.7
ndhD-ccsA_p3	CTCTCCATTAACCGGTTGGG	CGAAAGAGACGTGGGCATTA	55.0	50.0	58.0	58.0	917 bp	0.5	59.7
ndhD-ccsA_p4	GCGTAACCTCAAGAGAAGGG	TTGCAGCATCTACGGGATTC	55.0	50.0	58.0	58.0	1005 bp	0.5	59.5
ndhD-ccsA_p5	AAGGGTAGCCTATTCGTCGA	CGAAAGAGACGTGGGCATTA	50.0	50.0	57.9	58.0	1083 bp	0.5	59.5