Hotspot

ndhH-ndhA

SSC · 122700-123126 · 427 bp

Back to Dicranopteris

Pi 0.518

MarkerSeek score 78.30

Primer available yes

Label ndhH-ndhA

Feature Metadata

label_name: ndhH-ndhA
feature_type: igs
region: SSC
length_bp: 427
variable_sites: 197
pi: 0.518
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-ndhA_p1	GTTACGAGCTTCGGGGATTT	CGTACATAGGGGATGGGACT	50.0	55.0	58.0	58.0	1220 bp	0.5	59.8
ndhH-ndhA_p2	GTTACGAGCTTCGGGGATTT	TAGGGGATGGGACTCGATTC	50.0	55.0	58.0	58.0	1214 bp	0.5	59.8
ndhH-ndhA_p3	GTTACGAGCTTCGGGGATTT	ATGTTGGTGTTCCTGACCAC	50.0	50.0	58.0	58.0	1360 bp	0.5	59.7
ndhH-ndhA_p4	GTTACGAGCTTCGGGGATTT	CGCATAAAGCCAGGGGTATT	50.0	50.0	58.0	58.0	1059 bp	0.5	59.7
ndhH-ndhA_p5	GTTACGAGCTTCGGGGATTT	CTCGGAGTTTGCATGATCCA	50.0	50.0	58.0	58.0	1646 bp	0.5	59.6