primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	chlL	1	TATAGGGGGAAGCGGTAGTG	AGGGATTGACTCCACAGACA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.035397	113030	113977	2110-2110	0.500000	59.7
chlL_p2	chlL	2	TATAGGGGGAAGCGGTAGTG	AGATCGTAACTCCGAGAGCA	20	20	55.0	50.0	58.0	58.0	0.0	1.3	0.045188	113030	113977	1534-1534	0.500000	59.6
chlL_p3	chlL	3	AATAGAGGAATCTCCCCGGG	AGGGATTGACTCCACAGACA	20	20	55.0	50.0	58.0	58.0	15.8	0.0	0.061522	113030	113977	2337-2337	0.500000	59.5
chlL_p4	chlL	4	AATAGAGGAATCTCCCCGGG	AGATCGTAACTCCGAGAGCA	20	20	55.0	50.0	58.0	58.0	15.8	1.3	0.071313	113030	113977	1761-1761	0.500000	59.4
chlL_p5	chlL	5	GATAAACTCTTGAGGGGCGG	AGGGATTGACTCCACAGACA	20	20	55.0	50.0	58.0	58.0	2.5	0.0	0.075100	113030	113977	1852-1852	0.500000	59.4
chlN_p1	chlN	1	TGCTCTCGGAGTTACGATCT	CCCTGCACCACTCTGTATTC	20	20	50.0	55.0	58.0	58.0	4.7	0.0	0.069656	114307	115365	1402-1402	0.500000	59.4
chlN_p2	chlN	2	TGCTCTCGGAGTTACGATCT	GCCAATACGGGACTTGGTAG	20	20	50.0	55.0	58.0	58.1	4.7	3.5	0.090353	114307	115365	1913-1913	0.500000	59.3
chlN_p3	chlN	3	TGCTCTCGGAGTTACGATCT	TACAACGACATTCGAACGCT	20	20	50.0	45.0	58.0	57.9	4.7	0.0	0.094505	114307	115365	1487-1487	0.500000	59.2
chlN_p4	chlN	4	ACTCTCCCCTTTGAGTGTGA	CCCTGCACCACTCTGTATTC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.132992	114307	115365	1539-1539	0.500000	58.9
chlN_p5	chlN	5	TGCTCTCGGAGTTACGATCT	CATTACGTGGGGTTTGACCA	20	20	50.0	50.0	58.0	58.1	4.7	0.0	0.140916	114307	115365	1737-1737	0.500000	58.9
chlN-ycf1_p1	chlN-ycf1	1	AGCCCGATCTAGCCATTACT	GTCTCCGTACGGGACTATCA	20	20	50.0	55.0	58.0	58.0	0.6	31.7	0.057863	115366	116195	1313-1313	0.500000	59.5
chlN-ycf1_p2	chlN-ycf1	2	AGCCCGATCTAGCCATTACT	TCTCCGTACGGGACTATCAC	20	20	50.0	55.0	58.0	58.0	0.6	32.8	0.057863	115366	116195	1312-1312	0.500000	59.5
chlN-ycf1_p3	chlN-ycf1	3	AGCCCGATCTAGCCATTACT	TGTCTCCGTACGGGACTATC	20	20	50.0	55.0	58.0	58.0	0.6	35.0	0.057863	115366	116195	1314-1314	0.500000	59.5
chlN-ycf1_p4	chlN-ycf1	4	AGCCCGATCTAGCCATTACT	TGTGTTGGTCGAACAAGGAG	20	20	50.0	50.0	58.0	58.1	0.6	18.0	0.068800	115366	116195	1247-1247	0.500000	59.4
chlN-ycf1_p5	chlN-ycf1	5	AGCGTATGCATGAACCAGAG	TCTCCGTACGGGACTATCAC	20	20	50.0	55.0	58.1	58.0	10.2	32.8	0.095571	115366	116195	1330-1330	0.500000	59.2
ycf1_p1	ycf1	1	GGGACGAGTATTGGAACACC	CCCCATTCATTCCCGCAATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025992	117936	118055	1096-1096	0.500000	59.8
ycf1_p2	ycf1	2	GGGGACGAGTATTGGAACAC	CCCCATTCATTCCCGCAATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025992	117936	118055	1097-1097	0.500000	59.8
ycf1_p3	ycf1	3	GTGGGGACGAGTATTGGAAC	CCCCATTCATTCCCGCAATA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.025992	117936	118055	1099-1099	0.500000	59.8
ycf1_p4	ycf1	4	GGGACGAGTATTGGAACACC	AGGCTACTCCCCAAAATCCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.032868	117936	118055	875-875	0.500000	59.7
ycf1_p5	ycf1	5	GGGGACGAGTATTGGAACAC	AGGCTACTCCCCAAAATCCT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.032868	117936	118055	876-876	0.500000	59.7
ndhH_p1	ndhH	1	GGATCCGCCGAATCTCAAAT	CGTACATAGGGGATGGGACT	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.126887	121497	122699	2242-2242	0.500000	59.0
ndhH_p2	ndhH	2	GGATCCGCCGAATCTCAAAT	TAGGGGATGGGACTCGATTC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.126887	121497	122699	2236-2236	0.500000	59.0
ndhH_p3	ndhH	3	GGATCCGCCGAATCTCAAAT	CGCATAAAGCCAGGGGTATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.134508	121497	122699	2081-2081	0.500000	58.9
ndhH_p4	ndhH	4	GGATCCGCCGAATCTCAAAT	CATAGGGGATGGGACTCGAT	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.171588	121497	122699	2238-2238	0.500000	58.6
ndhH_p5	ndhH	5	GGATCCGCCGAATCTCAAAT	CTGCTCGGACATTCTCTCAC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.195847	121497	122699	2265-2265	0.500000	58.4
ndhH-ndhA_p1	ndhH-ndhA	1	GTTACGAGCTTCGGGGATTT	CGTACATAGGGGATGGGACT	20	20	50.0	55.0	58.0	58.0	9.7	0.0	0.031070	122700	123126	1220-1220	0.500000	59.8
ndhH-ndhA_p2	ndhH-ndhA	2	GTTACGAGCTTCGGGGATTT	TAGGGGATGGGACTCGATTC	20	20	50.0	55.0	58.0	58.0	9.7	0.0	0.031070	122700	123126	1214-1214	0.500000	59.8
ndhH-ndhA_p3	ndhH-ndhA	3	GTTACGAGCTTCGGGGATTT	ATGTTGGTGTTCCTGACCAC	20	20	50.0	50.0	58.0	58.0	9.7	0.0	0.038691	122700	123126	1360-1360	0.500000	59.7
ndhH-ndhA_p4	ndhH-ndhA	4	GTTACGAGCTTCGGGGATTT	CGCATAAAGCCAGGGGTATT	20	20	50.0	50.0	58.0	58.0	9.7	0.0	0.038691	122700	123126	1059-1059	0.500000	59.7
ndhH-ndhA_p5	ndhH-ndhA	5	GTTACGAGCTTCGGGGATTT	CTCGGAGTTTGCATGATCCA	20	20	50.0	50.0	58.0	58.0	9.7	0.0	0.048368	122700	123126	1646-1646	0.500000	59.6
ndhA_p1	ndhA	1	CGCCTAAGCAGGAACATCAT	ACTCGGACACATACCTCACA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.121718	123127	124806	2679-2679	0.500000	59.0
ndhA_p2	ndhA	2	CGCCTAAGCAGGAACATCAT	TTTCCAATCAATGACGGGCA	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.124453	123127	124806	2713-2713	0.500000	59.0
ndhA_p3	ndhA	3	CATGTAAGAGTGGAAGCCCC	ACTCGGACACATACCTCACA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.124882	123127	124806	2662-2662	0.500000	59.0
ndhA_p4	ndhA	4	ATCGCCAAAACGGTAACTGT	CCTCACCTTCCTTTGCACTT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.126231	123127	124806	2858-2858	0.500000	59.0
ndhA_p5	ndhA	5	CATGTAAGAGTGGAAGCCCC	TTTCCAATCAATGACGGGCA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.127617	123127	124806	2696-2696	0.500000	59.0
ndhI_p1	ndhI	1	TGTGAAAGGTTGCCATTCGA	AGACCTGTACAAGTTCCCGA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.031105	124922	125497	1648-1648	0.500000	59.8
ndhI_p2	ndhI	2	GCTGGTAGCGGGTTATCAAA	AGACCTGTACAAGTTCCCGA	20	20	50.0	50.0	58.0	58.0	2.4	0.0	0.038051	124922	125497	1607-1607	0.500000	59.7
ndhI_p3	ndhI	3	TGTGAAAGGTTGCCATTCGA	CCTCACCTTCCTTTGCACTT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.038853	124922	125497	1160-1160	0.500000	59.7
ndhI_p4	ndhI	4	GCTGGTAGCGGGTTATCAAA	CCTCACCTTCCTTTGCACTT	20	20	50.0	50.0	58.0	58.0	2.4	0.0	0.045798	124922	125497	1119-1119	0.500000	59.6
ndhI_p5	ndhI	5	GTGTGAAAGGTTGCCATTCG	AGACCTGTACAAGTTCCCGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.055847	124922	125497	1649-1649	0.500000	59.6
ndhG_p1	ndhG	1	AAGTGCAAAGGAAGGTGAGG	CCTACGCGATGAGGATAAGC	20	20	50.0	55.0	58.0	58.0	0.0	15.9	0.029145	125697	126293	899-899	0.500000	59.8
ndhG_p2	ndhG	2	ATCAAATGGCTTTGGGACGT	CCTACGCGATGAGGATAAGC	20	20	45.0	55.0	58.1	58.0	0.0	15.9	0.092672	125697	126293	1077-1077	0.500000	59.3
ndhG_p3	ndhG	3	TGTGAGGTATGTGTCCGAGT	CCTACGCGATGAGGATAAGC	20	20	50.0	55.0	58.1	58.0	0.0	15.9	0.096787	125697	126293	1291-1291	0.500000	59.2
ndhG_p4	ndhG	4	TGCCCGTCATTGATTGGAAA	CCTACGCGATGAGGATAAGC	20	20	45.0	55.0	58.1	58.0	8.9	15.9	0.099522	125697	126293	1257-1257	0.500000	59.2
ndhG_p5	ndhG	5	AAGTGCAAAGGAAGGTGAGG	CCCTGTTCAAGCATGACGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106424	125697	126293	866-866	0.500000	59.1
ndhG-psaC_p1	ndhG-psaC	1	TCGGGAACTTGTACAGGTCT	CCCACATGAGGAAGAAGAGC	20	20	50.0	55.0	58.0	58.0	11.4	11.2	0.040900	126294	127144	1869-1869	0.500000	59.7
ndhG-psaC_p2	ndhG-psaC	2	TCGGGAACTTGTACAGGTCT	GGGAGTGTTGGGAGCAATAG	20	20	50.0	55.0	58.0	58.0	11.4	0.0	0.049493	126294	127144	1519-1519	0.500000	59.6
ndhG-psaC_p3	ndhG-psaC	3	TCGGGAACTTGTACAGGTCT	AAAATAGGCGCGTATTCCGT	20	20	50.0	45.0	58.0	58.1	11.4	29.4	0.061339	126294	127144	1788-1788	0.500000	59.5
ndhG-psaC_p4	ndhG-psaC	4	TCGGGAACTTGTACAGGTCT	GACCCATACTGCGAGTTGTT	20	20	50.0	50.0	58.0	57.9	11.4	0.0	0.100132	126294	127144	1299-1299	0.500000	59.2
ndhG-psaC_p5	ndhG-psaC	5	TCGGGAACTTGTACAGGTCT	TTACAACCATCCCGGGGTAT	20	20	50.0	50.0	58.0	58.1	11.4	23.5	0.109285	126294	127144	1168-1168	0.500000	59.1
ndhD_p1	ndhD	1	TGGAGAATTTCAACGGCACA	CGAAAGAGACGTGGGCATTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.030318	127442	128950	2384-2384	0.500000	59.8
ndhD_p2	ndhD	2	TGGAGAATTTCAACGGCACA	TTGCAGCATCTACGGGATTC	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.072176	127442	128950	2291-2291	0.500000	59.4
ndhD_p3	ndhD	3	GAGCTTGTCCGACAGATGTT	CGAAAGAGACGTGGGCATTA	20	20	50.0	50.0	57.9	58.0	0.6	0.0	0.090637	127442	128950	2317-2317	0.500000	59.3
ndhD_p4	ndhD	4	TGGAGAATTTCAACGGCACA	AGGTCAACCTGAGAGAGTCC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.094100	127442	128950	1961-1961	0.500000	59.2
ndhD_p5	ndhD	5	TGGAGAATTTCAACGGCACA	GTCAACCTGAGAGAGTCCCT	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.094100	127442	128950	1959-1959	0.500000	59.2
ndhD-ccsA_p1	ndhD-ccsA	1	GCGTAACCTCAAGAGAAGGG	CGAAAGAGACGTGGGCATTA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.016123	128951	129232	1098-1098	0.500000	59.9
ndhD-ccsA_p2	ndhD-ccsA	2	TCACGCCCATCCTACATTTG	CGAAAGAGACGTGGGCATTA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041540	128951	129232	1182-1182	0.500000	59.7
ndhD-ccsA_p3	ndhD-ccsA	3	CTCTCCATTAACCGGTTGGG	CGAAAGAGACGTGGGCATTA	20	20	55.0	50.0	58.0	58.0	30.1	0.0	0.042969	128951	129232	917-917	0.500000	59.7
ndhD-ccsA_p4	ndhD-ccsA	4	GCGTAACCTCAAGAGAAGGG	TTGCAGCATCTACGGGATTC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.057981	128951	129232	1005-1005	0.500000	59.5
ndhD-ccsA_p5	ndhD-ccsA	5	AAGGGTAGCCTATTCGTCGA	CGAAAGAGACGTGGGCATTA	20	20	50.0	50.0	57.9	58.0	15.3	0.0	0.067553	128951	129232	1083-1083	0.500000	59.5
ccsA_p1	ccsA	1	CTCAGGTTGACCTCATCAGC	CCCTACTCGGAATAGCAGGA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.024557	129233	130198	1793-1793	0.500000	59.8
ccsA_p2	ccsA	2	CTCAGGTTGACCTCATCAGC	TCCCCTACTCGGAATAGCAG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.024557	129233	130198	1795-1795	0.500000	59.8
ccsA_p3	ccsA	3	CTCAGGTTGACCTCATCAGC	GTAACAAGCCGCTATGGTGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.039897	129233	130198	1547-1547	0.500000	59.7
ccsA_p4	ccsA	4	CTCTCCATTAACCGGTTGGG	CCCTACTCGGAATAGCAGGA	20	20	55.0	55.0	58.0	58.0	30.1	0.0	0.041588	129233	130198	2274-2274	0.500000	59.7
ccsA_p5	ccsA	5	CTCTCCATTAACCGGTTGGG	TCCCCTACTCGGAATAGCAG	20	20	55.0	55.0	58.0	58.0	30.1	0.0	0.041588	129233	130198	2276-2276	0.500000	59.7
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	GGAGATTTGAGCGAGTGTGT	CCCTACTCGGAATAGCAGGA	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.089256	130199	130459	636-636	0.500000	59.3
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	GGAGATTTGAGCGAGTGTGT	TCCCCTACTCGGAATAGCAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.089256	130199	130459	638-638	0.500000	59.3
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	GGAGATTTGAGCGAGTGTGT	GTAACAAGCCGCTATGGTGA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104596	130199	130459	390-390	0.500000	59.2
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	GGAGATTTGAGCGAGTGTGT	AGTAACAAGCCGCTATGGTG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.110230	130199	130459	391-391	0.500000	59.1
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	AGCGGTGTAGATTGTTGCAT	CCCTACTCGGAATAGCAGGA	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.117062	130199	130459	971-971	0.500000	59.1
trnP-GGG-rpl32_p1	trnP-GGG-rpl32	1	TCACCATAGCGGCTTGTTAC	GCTATGGCAGTTCCGAAGAA	20	20	50.0	50.0	58.0	58.0	8.3	0.0	0.042718	130787	132043	1697-1697	0.500000	59.7
trnP-GGG-rpl32_p2	trnP-GGG-rpl32	2	CACCATAGCGGCTTGTTACT	GCTATGGCAGTTCCGAAGAA	20	20	50.0	50.0	58.0	58.0	8.3	0.0	0.048351	130787	132043	1696-1696	0.500000	59.6
trnP-GGG-rpl32_p3	trnP-GGG-rpl32	3	TCACCATAGCGGCTTGTTAC	TGTCTCGGCAAGAAAACCAA	20	20	50.0	45.0	58.0	58.0	8.3	0.0	0.064103	130787	132043	1730-1730	0.500000	59.5
trnP-GGG-rpl32_p4	trnP-GGG-rpl32	4	TCACCATAGCGGCTTGTTAC	TTGTCTCGGCAAGAAAACCA	20	20	50.0	45.0	58.0	58.0	8.3	0.0	0.064103	130787	132043	1731-1731	0.500000	59.5
trnP-GGG-rpl32_p5	trnP-GGG-rpl32	5	CACCATAGCGGCTTGTTACT	TGTCTCGGCAAGAAAACCAA	20	20	50.0	45.0	58.0	58.0	8.3	0.0	0.069737	130787	132043	1729-1729	0.500000	59.4
rpl32-trnN-GUU_p1	rpl32-trnN-GUU	1	ACTTCCTCGCCAATGTTTGT	AGGGACTTGGAGGAGTGATC	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.145971	134203	134322	931-931	0.500000	58.8
rpl32-trnN-GUU_p2	rpl32-trnN-GUU	2	TGCTCTTTCATCATTCCCGG	AGGGACTTGGAGGAGTGATC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.169913	134203	134322	912-912	0.500000	58.6
rpl32-trnN-GUU_p3	rpl32-trnN-GUU	3	ACTTCCTCGCCAATGTTTGT	CACGAGACTCGGTTGTTGAA	20	20	45.0	50.0	58.0	58.2	0.0	27.4	0.170925	134203	134322	864-864	0.500000	58.6
rpl32-trnN-GUU_p4	rpl32-trnN-GUU	4	ACTTCCTCGCCAATGTTTGT	TCATCTCGTACGAGCGGATA	20	20	45.0	50.0	58.0	57.8	0.0	34.9	0.178071	134203	134322	835-835	0.500000	58.6
rpl32-trnN-GUU_p5	rpl32-trnN-GUU	5	TGCTCTTTCATCATTCCCGG	CACGAGACTCGGTTGTTGAA	20	20	50.0	50.0	58.0	58.2	0.0	27.4	0.194868	134203	134322	845-845	0.500000	58.4