Hotspot

ycf1

SSC · 120813-126149 · 5337 bp

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Pi 0.000937

MarkerSeek score 42.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5337
variable_sites: 12
pi: 0.000937
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACTTGAAACTTTTGAATGCTTCCT	AGGAACTGGCTTTGATACGT	33.3	45.0	57.8	56.8	243 bp	1.0	41.0
ycf1_p2	ACTTGAAACTTTTGAATGCTTCCT	AAGGAACTGGCTTTGATACGT	33.3	42.9	57.8	57.6	244 bp	1.0	41.0
ycf1_p3	ACTTGAAACTTTTGAATGCTTCCT	AGGAACTGGCTTTGATACGTT	33.3	42.9	57.8	57.6	243 bp	1.0	41.0
ycf1_p4	ACTTGAAACTTTTGAATGCTTCC	AGGAACTGGCTTTGATACGT	34.8	45.0	56.4	56.8	243 bp	1.0	41.0
ycf1_p5	TGAAACTTTTGAATGCTTCCT	AGGAACTGGCTTTGATACGT	33.3	45.0	54.2	56.8	240 bp	1.0	41.0