Hotspot

rps16

LSC · 4500-5658 · 1159 bp

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Pi 0.00104

MarkerSeek score 37.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1159
variable_sites: 3
pi: 0.00104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATTGAAGCTATGGGGCCCTA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2591-2592 bp	1.0	78.2
rps16_p2	TTGAAGCTATGGGGCCCTAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2590-2591 bp	1.0	78.2
rps16_p3	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2342-2343 bp	1.0	78.2
rps16_p4	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2341-2342 bp	1.0	78.2
rps16_p5	CGGTCTTCGAAATCACACCA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	2420-2421 bp	1.0	77.9