Hotspot

petB

LSC · 73733-75088 · 1356 bp

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Pi 0.000885

MarkerSeek score 51.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1356
variable_sites: 3
pi: 0.000885
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCAATTTGGTAGTTTGATCGTGG	TCCATACTTGTAGCAATAAAATCCT	43.5	32.0	58.3	56.3	1502-1510 bp	1.0	40.5
petB_p2	TGGATTTCTGTCCAATTTGGT	TCCATACTTGTAGCAATAAAATCCT	38.1	32.0	55.7	56.3	1513-1521 bp	1.0	40.5
petB_p3	TTGGTAGTTTGATCGTGGAA	TCCATACTTGTAGCAATAAAATCCT	40.0	32.0	54.6	56.3	1497-1505 bp	1.0	40.5
petB_p4	TTTGGTAGTTTGATCGTGGA	TCCATACTTGTAGCAATAAAATCCT	40.0	32.0	54.6	56.3	1498-1506 bp	1.0	40.5
petB_p5	TTTGGTAGTTTGATCGTGGAA	TCCATACTTGTAGCAATAAAATCCT	38.1	32.0	55.4	56.3	1498-1506 bp	1.0	40.5