Hotspot

ndhF

SSC · 108129-110423 · 2295 bp

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Pi 0.000915
MarkerSeek score 50.60
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2295
variable_sites
5
pi
0.000915
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TGAGAAATTCTATGGCTCGGA ACCAACTGTCACTTCTTGGT 42.9 45.0 56.5 56.9 2447-2452 bp 1.0 51.8
ndhF_p2 TGAGAAATTCTATGGCTCGGA ACCAACTGTCACTTCTTGGTT 42.9 42.9 56.5 57.6 2447-2452 bp 1.0 49.7
ndhF_p3 TTGAGAAATTCTATGGCTCGGA ACCAACTGTCACTTCTTGGT 40.9 45.0 57.2 56.9 2448-2453 bp 1.0 49.5
ndhF_p4 TGAGAAATTCTATGGCTCGGAT ACCAACTGTCACTTCTTGGT 40.9 45.0 57.0 56.9 2447-2452 bp 1.0 47.8
ndhF_p5 TTGAGAAATTCTATGGCTCGGA ACCAACTGTCACTTCTTGGTT 40.9 42.9 57.2 57.6 2448-2453 bp 1.0 47.4