Hotspot

ndhA

SSC · 116678-118774 · 2097 bp

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Pi 0.000382

MarkerSeek score 57.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2097
variable_sites: 2
pi: 0.000382
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACAAGTTGTGAGAGTTGTGAGA	CCGCCGGGTTTTATTAACTTG	40.9	47.6	57.5	57.9	2253-2255 bp	1.0	51.2
ndhA_p2	AGACAAGTTGTGAGAGTTGTGA	CCGCCGGGTTTTATTAACTTG	40.9	47.6	57.5	57.9	2255-2257 bp	1.0	51.2
ndhA_p3	TGAGACAAGTTGTGAGAGTTGT	CCGCCGGGTTTTATTAACTTG	40.9	47.6	57.5	57.9	2257-2259 bp	1.0	51.2
ndhA_p4	GACAAGTTGTGAGAGTTGTGAG	CCGCCGGGTTTTATTAACTTG	45.5	47.6	57.2	57.9	2254-2256 bp	1.0	48.7
ndhA_p5	GAGACAAGTTGTGAGAGTTGTG	CCGCCGGGTTTTATTAACTTG	45.5	47.6	57.2	57.9	2256-2258 bp	1.0	48.7