Hotspot

matK

LSC · 1806-3314 · 1509 bp

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Pi 0.00106

MarkerSeek score 52.70

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 4
pi: 0.00106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	ACGGCTTTCCCTATGTATACA	AAGTTTACCTGCTTCCGAGG	42.9	50.0	56.2	57.5	1738-1747 bp	1.0	54.7
matK_p2	ACGGCTTTCCCTATGTATACA	AGTTTACCTGCTTCCGAGGT	42.9	50.0	56.2	58.7	1737-1746 bp	1.0	53.2
matK_p3	ACGGCTTTCCCTATGTATACA	ACCTGCTTCCGAGGTATCTA	42.9	50.0	56.2	57.2	1732-1741 bp	1.0	52.5
matK_p4	ACGGCTTTCCCTATGTATACA	TACCTGCTTCCGAGGTATCT	42.9	50.0	56.2	57.2	1733-1742 bp	1.0	52.5
matK_p5	CGGCTTTCCCTATGTATACATCA	AAGTTTACCTGCTTCCGAGG	43.5	50.0	57.9	57.5	1737-1746 bp	1.0	51.6