Hotspot

rps4-trnT-UGU

LSC · 44955-45560 · 606 bp

Back to Dicliptera

Pi 0.00333

MarkerSeek score 54.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 5
pi: 0.00333
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TTTCTAAGATCGCTTCCGGC	ATATAGGCCCGCTTAGCTCA	50.0	50.0	58.1	58.1	780-792 bp	1.0	80.1
rps4-trnT-UGU_p2	GGAGCGTGGTTGGTTTCTAA	ATATAGGCCCGCTTAGCTCA	50.0	50.0	58.1	58.1	793-805 bp	1.0	79.7
rps4-trnT-UGU_p3	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	764-776 bp	1.0	79.6
rps4-trnT-UGU_p4	GGAGCGTGGTTGGTTTCTAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	777-789 bp	1.0	79.2
rps4-trnT-UGU_p5	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	732-744 bp	1.0	78.8